Human rhinovirus 17 (ATCC® VR-1663)

Classification: Picornaviridae, Rhinovirus  /  Product Format: frozen

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Classification Picornaviridae, Rhinovirus
Agent Human rhinovirus 17
Strain 33342
Applications
Respiratory research
Biosafety Level 2
Isolation
This product is a mycoplasma free preparation derived from NIAID Research Reference Reagent V-106-003-021 (ATCC VR-1127). This item replaces ATCC VR-1127 and ATCC VR-282 , which will be removed from the catalog on depletion of inventory. Strain 33342 is the prototype strain for human rhinovirus 17 and it has been fully sequenced (GenBank: EF173423.1).
Product Format frozen
Storage Conditions -70°C or colder
Genome Sequenced Strain

Yes

Comments
This product is a mycoplasma free preparation derived from NIAID Research Reference Reagent V-106-003-021 (ATCC VR-1127). This item replaces ATCC VR-1127 and ATCC VR-282 , which will be removed from the catalog on depletion of inventory. Strain 33342 is the prototype strain for human rhinovirus 17 and it has been fully sequenced (GenBank: EF173423.1).
Effect on Host
Typical rhinovirus CPE in cell culture, including rounding and sloughing of infected cells.
Typical rhinovirus CPE in cell culture, including rounding and sloughing of infected cells.
Growth Medium Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
Recommended Host
Production Host:H1-HeLa (ATCC CRL-1958)

Production Host: H1HeLa (ATCC® CRL-1958™)

Alternate Hosts:WI-38 (ATCC CCL-75 ), HeLa (ATCC CCL-2 ), KB (ATCC CCL-17 ) and other human cells.
Growth Conditions
For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluence using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 33.0°C in a humidified, 5% CO2 atmosphere for 2-7 days with rocking, until CPE are well advanced in 100% of the culture. Harvest cells and culture fluids; cell debris can be removed by centrifugation if desired.
Effect on Host
Typical rhinovirus CPE in cell culture, including rounding and sloughing of infected cells.
Typical rhinovirus CPE in cell culture, including rounding and sloughing of infected cells.
Name of Depositor NIAID, ATCC
Source
Presumed from throat swab from adult with upper respiratory illness, North Carolina, 1959; derived from ATCC VR-1127 following treatment to remove mycoplasma contamination.
References

Fenters JD, et al. Propagation of Rhinovirus on WI-38 cell monolayers in rolling bottles. Appl. Microbiol. 15: 1460-1464, 1967.

Tapparel C, et al. New complete genome sequences of human rhinoviruses shed light on their phylogeny and genomic features. BMC Genomics 8: 224, 2007. PubMed: 17623054

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation