Human respiratory syncytial virus (ATCC® VR-1580)

Classification: Paramyxoviridae, Pneumovirus  /  Product Format: frozen 1 mL per vial

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Classification Paramyxoviridae, Pneumovirus
Deposited As Respiratory syncytial virus
Agent Human respiratory syncytial virus
Strain 18537
Applications
Respiratory research
Positive for Mycoplasma No
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1580 images
Comments The early passage history of this item is unknown.
Effect on Host
CPE, massive syncytia formation and sloughing
Recommended Host
HEp-2 (ATCC® CCL-23™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-7 days
Effect on Host
CPE, massive syncytia formation and sloughing
Name of Depositor RM Chanock, J Crowe, National Institute of Allergy and Infectious Diseases; ATCC®
Special Collection ATCC
Source Respiratory secretions from child with acute respiratory disease seen at Children’s Hospital of the District of Columbia, Washington, DC, 1962. Treated ATCC® VR-1401™ to remove mycoplasma at ATCC®.
References

Coates HV, et al. An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test. Am. J. Epidemiol. 83: 299-313, 1966. PubMed: 5933417

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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