Human adenovirus 4 (ATCC® VR-1572)

Classification: Adenoviridae, Mastadenovirus, Human adenovirus E  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

View Permits

Classification Adenoviridae, Mastadenovirus, Human adenovirus E
Agent Human adenovirus 4
Strain RI-67
Applications
Respiratory research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Derived by adaptation from HEK cell product ATCC® VR-1081™ to HeLa-229 cells (ATCC® CCL-2.1™). ATCC® VR-1081™ and ATCC® VR-1572™ have not been compared for sequence or infectivity in primary cell culture and tissue culture.
Effect on Host CPE, cell rounding and degeneration
Recommended Host HeLa-229 (ATCC® CCL-2.1™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-7 days
Effect on Host CPE, cell rounding and degeneration
Name of Depositor WP Rowe, National Institute of Allergy and Infectious Diseases; ATCC®
Special Collection ATCC
Source Throat washings of patient, Fort Leonard Wood, Missouri, 1952-1953
References

Huebner RJ, et al. Adenoidal-pharyngeal-conjunctival agents: a newly recognized group of common viruses of the respiratory system. N. Engl. J. Med. 251: 1077-1086, 1954. PubMed: 13223958

Werner JH, Hilleman MR. Recovery of new agent from patients with acute respiratory illness. Proc. Soc. Exp. Biol. Med. 85: 183-188, 1954. PubMed: 13134329

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation