Human herpesvirus 1 (ATCC® VR-1493)

Classification: Herpesviridae, Simplexvirus  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

View Permits

Classification Herpesviridae, Simplexvirus
Agent Human herpesvirus 1
Strain KOS
Common Name Herpes simplex virus 1
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments VR-1493™ was derived from VR-1487™ following treatment with a mycoplasma removal agent to remove mycoplasma contamination. Antigenically cross-reactive with Human herpesvirus 2.
Effect on Host
CPE, syncytial formation, cell rounding, clumping, and sloughing
Recommended Host
Vero (ATCC® CCL-81™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-3 days
Effect on Host
CPE, syncytial formation, cell rounding, clumping, and sloughing
Name of Depositor PA Schaffer, University of Pennsylvania
Special Collection NCRR Contract
Source Lip lesion of human with cold sore
References

Smith KO. Relationship between the envelope and the infectivity of herpes simplex virus. Proc. Soc. Exp. Biol. Med. 115: 814-816, 1964. PubMed: 14155835

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation