Human herpesvirus 3 (ATCC® VR-1367)

Classification: Herpesviridae, Varicellovirus  /  Product Format: frozen 1 mL per vial

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Classification Herpesviridae, Varicellovirus
Deposited As Varicella-Zoster
Agent Human herpesvirus 3
Strain Ellen
Common Name Varicella zoster virus
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions Liquid nitrogen vapor phase
Comments
VR-1367™ is a mycoplasma-free item derived at ATCC® from VR-586™ by three passages in the presence of a mycoplasma  removal agent.
Effect on Host
CPE, syncytial rounding and sloughing
Recommended Host
MRC-5 (ATCC® CCL-171™)
Growth Conditions
Temperature: 36°C
Recommendations For Infection: This product is produced by co-cultivation of virus with fresh host cells. Seed culture vessels at 1 x 105 cells per cm2. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.01) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate for 24 hours at 36°C in a humidified 5% CO2 atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation.
Incubation: 4-7 days
Effect on Host
CPE, syncytial rounding and sloughing
Name of Depositor EI Rosanoff, Wyeth Laboratories, Inc.
Special Collection NCRR Contract
Source Vesicular fluid from child with chickenpox, Georgia. Derived from VR-586™. Treated to remove Mycoplasma hominis at ATCC®.
References

Brunell PA, Casey HL. Crude tissue culture antigen for determination of Varicella-Zoster complement fixing antibody. Public Health Rep. 79: 839-842, 1964. PubMed: 14213793

Brunell PA. Transmission of chickenpox in a school setting prior to the observed exanthem. Am. J. Dis. Child. 143: 1451-1452, 1989. PubMed: 2556025

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation