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Human herpesvirus 3

VR-1367

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Human herpesvirus 3 strain Ellen is a mycoplasma-free strain derived from ATCC VR-586 by three passages in the presence of a mycoplasma removal agent. The strain was originally isolated from the vesicular fluid from a child with the chickenpox in Georgia, United States, and can be propagated in MRC-5 cells (ATCC CCL-171). Human herpesvirus 3 has applications in infectious disease research.
Product category
Viruses
Product type
Animal virus
Classification
Herpesviridae, Varicellovirus
Strain designation
Ellen
Common name
Varicella zoster virus
Genome sequenced strain
Yes
Isolation source
Vesicular fluid from child with chickenpox, Georgia. Derived from ATCC VR-586. Treated to remove Mycoplasma hominis.
Geographical isolation
United States; Georgia
Product format
Frozen
Storage conditions
Vapor phase of liquid nitrogen
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

Characteristics

Comments
ATCC VR-1367 is a mycoplasma-free item derived at ATCC from ATCC VR-586 by three passages in the presence of a mycoplasma removal agent.
Mycoplasma contamination
Not detected

Handling information

Host
Effects
cell rounding; cell sloughing; CPE; syncytia
Complete medium
EMEM (ATCC 30-2003) + 2% FBS (ATCC 30-2020)
Temperature
36°C
Atmosphere
95% Air, 5% CO2
Incubation
4-7 days
Recommendation for infection
This product is produced by co-cultivation of virus with fresh host cells. Seed culture vessels at 1 x 105 cells per cm2. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.01) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate for 24 hours at 36°C in a humidified 5% CO2 atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation.
Handling notes
Passage the virus by co-infection of fresh cells. Thaw ampoule in 37°C water bath. Use virus to co-infect cells in a T25 flask for first passage. Multiple passages may enhance the titer. Preserve as you would live cells.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
CPE, Cytopathic effect
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum

Quality control specifications

Volume
1.0 mL
Verification method
Whole-genome Sequencing

History

Deposited as
Varicella-Zoster
Depositors
EI Rosanoff, Wyeth Laboratories, Inc.
Type of isolate
Human
Special collection
NCRR Contract

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Brunell PA, Casey HL. Crude tissue culture antigen for determination of Varicella-Zoster complement fixing antibody. Public Health Rep. 79: 839-842, 1964. PubMed: 14213793

Brunell PA. Transmission of chickenpox in a school setting prior to the observed exanthem. Am. J. Dis. Child. 143: 1451-1452, 1989. PubMed: 2556025

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