This strain is recommended by ATCC for use in the tests described in Fed. Spec. A-A-1438 where only the taxon is specified.
The host range includes many mammalian and avian cells, e.g.,HeLa S3 (ATCC CCL-2.2)
and BS-C-1 (ATCC CCL-26)
This tissue culture adapted WR strain of vaccinia is derived from ATCC VR-119
and has been shown to serve as an effective expression vector for foreign genes (Mackett et al., J. Virol., 1984)
Dr. Moss received the WR strain in 1966 from Dr. Salzman who obtained the virus as VR-119 from the ATCC. The tissue culture adapted strain WR has been widely distributed and used in constructing vectors for gene expression (J. Gen Virol, 1986).
Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4).
Cell associated virus from infected HeLa cells
Effect on Host
Yes, in vitro effects: Cytopathic effects (localized enlarged, rounded cells which eventually extend throughout the monolayer)
CPE, cell rounding and sloughing