Human adenovirus 11 (ATCC® VR-12)

Classification: Adenoviridae, Mastadenovirus, Human adenovirus B  /  Product Format: frozen

Classification Adenoviridae, Mastadenovirus, Human adenovirus B
Agent Human adenovirus 11
Strain Slobitski
Applications
Respiratory research
Biosafety Level 2
Isolation
feces, human
Massachusetts, United States
Product Format frozen
Storage Conditions -70°C or colder
Comments
Reference Strain: Pereira, H.G. et al., Virology 20: 613, 1963. Efforts at ATCC to detect polio by aby-agn rxn in VR-12 have yielded negative results. Contains soluble group-specific CF antigen
Agglutinates rhesus RBC. Weakly oncogenic.
Effect on Host
Yes, in vitro effects: Cytopathic effects, refractile rounding, clumping and sloughing
CPE, refractile rounding, clumping and sloughing
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host
KB cells (ATCC CCL-17)
Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37°C
Recommendation for Infection: 2-4 days; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent).
Effect on Host
Yes, in vitro effects: Cytopathic effects, refractile rounding, clumping and sloughing
CPE, refractile rounding, clumping and sloughing
Name of Depositor M Schaeffer, DA Stevens
Special Collection ATCC
Source
feces, human
Massachusetts, United States
References

Hartley JW, et al. Additional serotypes of the APC virus group. Proc. Soc. Exp. Biol. Med. 91: 260-262, 1956. PubMed: 13297771

Melendez L, et al. Clinical associations of enteric viruses with particular reference to agents exhibiting properties of the ECHO group. Ann. N.Y. Acad. Sci. 67: 311-325, 1957. PubMed: 13411969

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016

can also be grown on other human cell lines

feces from patient with paralytic poliomyelitis, Massachusetts. [Type 1 poliovirus also isolated.]

Cross References

Nucleotide (GenBank) : U52569 Human adenovirus type 11 virus-associated RNA pre-terminal protein (pTP) and 52,55K protein genes, partial cds.