Human adenovirus 11 (ATCC® VR-12)

Classification: Adenoviridae, Mastadenovirus, Human mastadenovirus B  /  Product Format: frozen 1.0 mL per vial

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Classification Adenoviridae, Mastadenovirus, Human mastadenovirus B
Deposited As Adenovirus type 11
Agent Human adenovirus 11
Strain Slobitski
Applications
Respiratory research
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Images ATCC VR-12 micrograph of host effects
Comments
Agglutinates rhesus RBC.  Weakly oncogenic.  Contains soluble group-specific CF antigen.  Efforts at ATCC® to detect polio by antibody-antigen reaction in VR-12™ have yielded negative results.
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
KB cells (ATCC® CCL-17™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, cell rounding and sloughing
Name of Depositor M Schaeffer, DA Stevens
Special Collection ATCC
Source Feces, human, Massachusetts, United States
References

Rowe WP, Hartley JW, Huebner RJ. Additional serotypes of the APC virus group. Proc. Soc. Exp. Biol. Med. 91: 260-262, 1956. PubMed: 13297771

Kibrick S, Melendez L, Enders JF. Clinical associations of enteric viruses with particular reference to agents exhibiting properties of the ECHO group. Ann. NY Acad. Sci. 67: 311-325, 1957. PubMed: 13411969

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016

Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828

Feces from patient with paralytic poliomyelitis, Massachusetts. [Type 1 poliovirus also isolated.]

Cross References

Nucleotide (GenBank) : U52569 Human adenovirus type 11 virus-associated RNA pre-terminal protein (pTP) and 52,55K protein genes, partial cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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