P388D1 (IL-1) (ATCC® TIB-63)

Organism: Mus musculus, mouse  /  Cell Type: methylcholanthrene induced  / 

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Organism Mus musculus, mouse
Cell Type methylcholanthrene induced
Product Format frozen
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain DBA/2
Applications
The cells are negative for surface immunoglobulin (sIg-).
Tested and found negative for ectromelia virus (mousepox).
Receptor Expression
complement (C3)
Genes Expressed
interleukin-1 (interleukin 1, IL-1 or LAF) induced by LPS or phorbol myristic acid (PMA, TPA); lysozyme
Cellular Products
interleukin-1 (interleukin 1, IL-1 or LAF) induced by LPS or phorbol myristic acid (PMA, TPA); lysozyme
Comments
These cells phagocytose zymosan and latex beads.
They are active in antibody dependent cell mediated cytotoxicity systems.
The cells are negative for surface immunoglobulin (sIg-).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 3 times per week
Subcultures are prepared by scraping. Remove old medium, add fresh dislodge the cells and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
Name of Depositor S Mizel
References

Mizel SB. Physicochemical characterization of lymphocyte-activating factor (LAF). J. Immunol. 122: 2167-2172, 1979. PubMed: 312860

Mizel SB. Biochemical and biological characterization of lymphocyte-activating factor (LAF) produced by the murine macrophage cell line, P388D1. Ann. N.Y. Acad. Sci. 332: 539-549, 1979. PubMed: 231409

Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922

Koren HS, et al. Identification of macrophage-like characteristics in a cultured murine tumor line. J. Immunol. 114: 894-897, 1975. PubMed: 1167564

. Manual of macrophage methodology. New York: Marcel Dekker; 1981.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Mizel SB. Physicochemical characterization of lymphocyte-activating factor (LAF). J. Immunol. 122: 2167-2172, 1979. PubMed: 312860

Mizel SB. Biochemical and biological characterization of lymphocyte-activating factor (LAF) produced by the murine macrophage cell line, P388D1. Ann. N.Y. Acad. Sci. 332: 539-549, 1979. PubMed: 231409

Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922

Koren HS, et al. Identification of macrophage-like characteristics in a cultured murine tumor line. J. Immunol. 114: 894-897, 1975. PubMed: 1167564

. Manual of macrophage methodology. New York: Marcel Dekker; 1981.