P388D1 (IL-1) (ATCC® TIB-63)

Organism: Mus musculus, mouse  /  Cell Type: methylcholanthrene induced  / 

Permits and Restrictions

View Permits

Organism Mus musculus, mouse
Cell Type methylcholanthrene induced
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Strain DBA/2
Applications
The cells are negative for surface immunoglobulin (sIg-).
Tested and found negative for ectromelia virus (mousepox).
Receptor Expression
complement (C3)
Genes Expressed
interleukin-1 (interleukin 1, IL-1 or LAF) induced by LPS or phorbol myristic acid (PMA, TPA); lysozyme
Comments
These cells phagocytose zymosan and latex beads.
They are active in antibody dependent cell mediated cytotoxicity systems.
The cells are negative for surface immunoglobulin (sIg-).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 3 times per week
Subcultures are prepared by scraping. Remove old medium, add fresh dislodge the cells and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
Name of Depositor S Mizel
References

Mizel SB. Physicochemical characterization of lymphocyte-activating factor (LAF). J. Immunol. 122: 2167-2172, 1979. PubMed: 312860

Mizel SB. Biochemical and biological characterization of lymphocyte-activating factor (LAF) produced by the murine macrophage cell line, P388D1. Ann. N.Y. Acad. Sci. 332: 539-549, 1979. PubMed: 231409

Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922

Koren HS, et al. Identification of macrophage-like characteristics in a cultured murine tumor line. J. Immunol. 114: 894-897, 1975. PubMed: 1167564

. Manual of macrophage methodology. New York: Marcel Dekker; 1981.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Mizel SB. Physicochemical characterization of lymphocyte-activating factor (LAF). J. Immunol. 122: 2167-2172, 1979. PubMed: 312860

Mizel SB. Biochemical and biological characterization of lymphocyte-activating factor (LAF) produced by the murine macrophage cell line, P388D1. Ann. N.Y. Acad. Sci. 332: 539-549, 1979. PubMed: 231409

Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922

Koren HS, et al. Identification of macrophage-like characteristics in a cultured murine tumor line. J. Immunol. 114: 894-897, 1975. PubMed: 1167564

. Manual of macrophage methodology. New York: Marcel Dekker; 1981.