EL4.BU.1.OUAr.1.1 (ATCC® TIB-41)

Organism: Mus musculus, mouse  /  Cell Type: T lymphocyte  /  Disease: lymphoma

Organism Mus musculus, mouse
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Strain C57BL/6N
Storage Conditions liquid nitrogen vapor phase
Derivation
This line was derived from the EL4 cell line (see ATCC TIB-39).
Antigen Expression
H-2b; Thy-1.2
Comments
This line was derived from the EL4 cell line (see ATCC TIB-39).
The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine and 1 mM ouabain.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2-3 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.

Medium Renewal: Every 2 to 3 days


Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor R Hyman
References

Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.

Basic Documentation
References

Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.