Mouse ES Cell Basal Medium (ATCC® SCRR-2011)

Product Format 500 mL
Storage Conditions Store medium at 2°C to 8°C in the dark when not in use.
Applications General culture conditions to maintain undifferentiated,  pluripotent mouse ES cells are now well established, and  incremental improvements, such as the development of  Mouse ES Cell Basal Medium, are key to the ongoing  success of stem cell research.

Mouse ES Cell Basal Medium is a sterile liquid tissue culture  medium containing phenol red that has been optimized to  support the undifferentiated self-renewal cycle related to  mouse embryonic stem cell growth.

The medium contains a stable glutamine dipeptide, providing  a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell  Qualified Fetal Bovine Serum (FBS) or a serum replacement  designed for mouse ES cells. This medium can support the growth of mouse ES cells on  either a mitotically inactivated layer of mouse embryonic  fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.

Biosafety Level 1
Quality Control Specifications

Sterility tests are performed on each lot of medium using current USP methods. Each lot of medium is also checked for its ability to support the growth of several different cell lines using both sequential subcultures and plating efficiency. Additional test results are listed in the Certificate of Analysis.

Test*

Specification

pH

7.1 to 7.5

Osmolality

260 to 285 mOsm/kg

Sterility Testing

Negative for bacteria, fungi, yeast

Appearance

Clear red solution

Cell testing (ES-D3 ES cells)

Rate of proliferation and morphology