HFF-1 IRR (ATCC® SCRC-1041.1)

Organism: Homo sapiens, human  /  Cell Type: Fibroblast  /  Tissue: Skin, foreskin  / 

Organism Homo sapiens, human
Tissue Skin, foreskin
Cell Type Fibroblast
Product Format frozen
Morphology Fibroblast
Culture Properties Adherent
Biosafety Level 1

[Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office (2007). The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm]

 

 

 

 

 

Age newborn
Gender Male
Applications
Irradiated cells for use as feeder layer. These cells are provided to be used as feeder cells to support the growth of stem cells in the undifferentiated state.
Storage Conditions liquid nitrogen vapor phase
Clinical Data
Male
Comments

Once the feeder cells have attached, the culture medium can be changed to accommodate the cells to be supported. It is recommended that the feeder cells be plated 24 hours before use at 5 to 6 X 106/T75 in order to obtain a 100% confluent monolayer for stem cells growth.

These cells have been growth-arrested by irradiation with 6,000 rads.  The cells will begin to deteriorate 2 weeks after plating and may no longer support the growth of other cells. We recommend that you do not keep the cells in culture for longer than 2 weeks.


Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).
    Culture Conditions
    Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
    Name of Depositor ATCC
    Year of Origin 2003
    References

    Amit M, et al. Human feeder layers for human embryonic stem cells. Biol. Reprod. 68: 2150-2156, 2003. PubMed: 12606388

    Hovatta O, et al. A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells. Hum. Reprod. 18: 1404-1408, 2003. PubMed: 12832363

    Andrews P, et al. Human embryonic fibroblast feeder cells. International Patent Application WO 03/078611 A1

    Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

    Basic Documentation
    References

    Amit M, et al. Human feeder layers for human embryonic stem cells. Biol. Reprod. 68: 2150-2156, 2003. PubMed: 12606388

    Hovatta O, et al. A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells. Hum. Reprod. 18: 1404-1408, 2003. PubMed: 12832363

    Andrews P, et al. Human embryonic fibroblast feeder cells. International Patent Application WO 03/078611 A1

    Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.