SK-MEL-28 (ATCC® HTB-72)

Organism: Homo sapiens, human  /  Cell Type: Melanoma  /  Tissue: skin  /  Disease: malignant melanoma

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Organism Homo sapiens, human
Tissue skin
Cell Type Melanoma
Product Format frozen
Morphology polygonal
Culture Properties adherent
Biosafety Level 1
Disease malignant melanoma
Age 51 years adult
Gender male
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 90; range = 81 to 96. This is a hypotetraploid human cell line with the modal chromosome number of 90, occurring in 50% of cells. The rate of cells with a higher ploidy was 3.6%. This cell line has a very complex karyotype. There were 18 or more marker chromosomes that were common to most cells. Markers der(1)t(1;2) (p36.1;q21), del(1) (q2101) and several others had two copies per cell and t(2p14q), t(3q7p) and others had a single copy per cell. The Y/autosome translocation marker was identified as der(20)t(Y;20) (q11.23;q13.3) and had two copies per cell. The inclusion of a short segment of the euchromatic Yq11.23 was confirmed by the Southern blot DNA analysis. There were two normal X chromosomes per cell; Normal Y, N1 and N11 were absent; N19 had five or more copies per cell.
Derivation
Clinical Data
male
Antigen Expression
Antigen expression: Blood Type A; Rh+; HLA A11, A26, B40, DRw4
Tumorigenic Yes
Effects
Yes, in nude mice; forms malignant melanoma (large round cell type)
Comments
This is one of a very extensive series of melanoma lines (see also ATCC HTB-70, ATCC HTB-71 and ATCC HTB-73) isolated by T. Takahashi and associates.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9,12
D5S818: 11,13
D7S820: 10,9.3
THO1: 7
TPOX: 8,12
vWA: 16,19
Isoenzymes
AK-1, 1-2
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
PGM3, 1
Name of Depositor T Takahashi
References

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619

Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999.

Cross References

Nucleotide (GenBank) : D55649 Human mRNA for alpha mannosidase II isozyme, complete cds.

Nucleotide (GenBank) : L28821 Homo sapiens alpha mannosidase II isozyme mRNA, complete cds.

Nucleotide (GenBank) : NM_006122 Homo sapiens mannosidase, alpha, class 2A, member 2 (MAN2A2), mRNA.

Nucleotide (GenBank) : AW067366 EST00008 RT-PCR products human melanoma Homo sapiens cDNA clone Mel8, mRNA sequence.

Nucleotide (GenBank) : AW067367 EST00009 RT-PCR products human melanoma Homo sapiens cDNA clone Mel10, mRNA sequence.

Nucleotide (GenBank) : AW067368 EST00010 RT-PCR products human melanoma Homo sapiens cDNA clone Mel12, mRNA sequence.

Nucleotide (GenBank) : AW067369 EST00011 RT-PCR products human melanoma Homo sapiens cDNA clone Mel15, mRNA sequence.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the cells subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with the Office of Technology Development, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Contact Tingting Zhang-Kharas, Direct Phone: 646-888-1083, Reception: 646-888-1080, Email: zhangkht@mskcc.org

References

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212

Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619

Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999.