CAMA-1 (ATCC® HTB-21)

Organism: Homo sapiens, human  /  Tissue: mammary gland/breast; derived from metastatic site: pleural effusion  /  Disease: adenocarcinoma

Organism Homo sapiens, human
Tissue
mammary gland/breast; derived from metastatic site: pleural effusion
Product Format frozen
Morphology epithelial
Culture Properties Adherent patches of epithelial cells; compact, multilayered colonies, rarely become confluent
Biosafety Level 1
Disease adenocarcinoma
Age 51 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 80; range = 68 to 83.
This is a hypertriploid human cell line with the modal chromosome number of 80 occurring in 44% of a total of 88 cells examined. The rate of polyploid cells was 3.5%. Karyotypes of this cell line were generally uniform and stable. There were 12-13 marker chromosomes per cell, 11 of which were found in all cells, and 7 of which were mostly paired. Among the markers were paired mar(1qter--q21::?::6p11.2--6pter), t(3q::?), and i(16q); single der(12)t(12;?)(q24:?); and 8-9 others. Double minutes occurred in some cells; however, they were present as only one or two copies per cell. Structurally normal N16 was absent and N8 occurred in only a few cells. Paired normal X chromosomes were present in every cell.
Derivation
The CAMA-1 line was established by J. Fogh at Sloan-Kettering in 1975 from cells in the pleural effusion of a patient with carcinoma of the breast.
Clinical Data
51 years
Caucasian
female
Antigen Expression

Blood type O; Rh +; HLA A10, A11, B12, B18

Tumorigenic Yes
Effects
Yes, in nude mice
Comments
An ampule frozen in May of 1978 at passage 21 was provided to the ATCC in June of 1991.
The cells form poorly differentiated grade III tumors consistent with breast carcinoma.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 12,13
D7S820: 8,11
THO1: 8,9.3
TPOX: 8
vWA: 15
Isoenzymes
AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 1
PGM3, 1
Name of Depositor J Fogh
Passage History
An ampule frozen in May of 1978 at passage 21 was provided to the ATCC in June of 1991.
Year of Origin 1975
References

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Basic Documentation
References

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871