F1A3-23 (ATCC® HB-8207™) Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) / Cell Type: hybridoma: B lymphocyte General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) Cell Type hybridoma: B lymphocyte Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Derivation Animals were immunized with purified CSF-I.Spleen cells were fused with Sp2/0-Ag14 myeloma cells.CSF-I was purified from medium conditioned by MIA PaCa-2 (ATCC CRL-1420). Genes Expressed immunoglobulin; monoclonal antibody; against human colony stimulating factor subclass I (CSF-I) Tumorigenic Yes Comments Animals were immunized with purified CSF-I.Spleen cells were fused with Sp2/0-Ag14 myeloma cells.CSF-I was purified from medium conditioned by MIA PaCa-2 (ATCC CRL-1420). Complete Growth Medium HB101 medium (Hana Biologics), 99%; heat-inactivated fetal bovine serum, 1% Subculturing Medium Renewal: Twice per weekCultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Isotype IgG1 Name of Depositor Amgen, Inc. References Nicolson M. Hybridoma tumor cell lines and their monoclonal antibodies to human colony stimulating factor subclass number 1. US Patent 4,504,586 dated Mar 12 1985 . . Hybridoma 2: 137, 1983. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References Nicolson M. Hybridoma tumor cell lines and their monoclonal antibodies to human colony stimulating factor subclass number 1. US Patent 4,504,586 dated Mar 12 1985 . . Hybridoma 2: 137, 1983.