Hep 3B2.1-7 [Hep 3B, Hep-3B, Hep3B] (ATCC® HB-8064)

Organism: Homo sapiens, human  /  Tissue: Liver  /  Disease: hepatocellular carcinoma

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Organism Homo sapiens, human
Tissue Liver
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2  [Cells contain Hepatitis B]
Disease hepatocellular carcinoma
Age 8 years juvenile
Gender male
Ethnicity Black
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 60 with a subtetraploid mode of 82; has a rearranged chromosome 1 (Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983)
Clinical Data
8 years juvenile
Black
male
Genes Expressed
alpha fetoprotein (alpha-fetoprotein); hepatitis B surface antigen (HBsAg); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin;,alpha1 antichymotrypsin (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen; complement (C3, C4); C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein);,alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-
Cellular Products
Cellular products: alpha fetoprotein (alpha-fetoprotein); hepatitis B surface antigen (HBsAg); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin;
alpha1 antichymotrypsin (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen; complement (C3, C4); C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein);
alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein); Gc globulin
Tumorigenic Yes
Effects
Yes, forms tumors in nude mice
Comments
This line contains an integrated hepatitis B virus genome.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 8
D13S317: 12,14
D16S539: 10
D5S818: 13
D7S820: 8,10
THO1: 6,7
TPOX: 9
vWA: 17
Name of Depositor Wistar Institute
References

Knowles BB, et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960

Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983

Aden DP, et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137

Darlington GJ, et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851

Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Knowles BB, et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960

Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983

Aden DP, et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137

Darlington GJ, et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851

Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983