Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Medium Renewal: Every 2 to 3 days
This line has carried by passage as ascites in pristane primed (0.5 ml, 7 days prior to injection of cells) mice.
The mice are injected with 0.3 to 0.5 ml of cells, and the ascites is collected 7 to 10 days later.
Recent information from the donor indicates that the cells are readily grown in culture using the above medium.
Cultures can be maintained by addition or replacement of fresh medium.
Start cultures at 5 X 10 exp4 cells/ml and do not allow the cells to exceed 8 X 10 exp5 cells/ml.