ILB1-H6 [ILB1-H6A1B] (ATCC® HB-10219)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts specifically with IL-1 beta and does not react with IL-1 alpha or with acidic or basic fibroblast growth factor.
Derivation
Animals were immunized with purified recombinant human IL-1 beta (17500 dalton form).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human interleukin 1 beta (interleukin-1 beta, IL-1 beta)
Cellular Products
immunoglobulin; monoclonal antibody; against human interleukin 1 beta (interleukin-1 beta, IL-1 beta)
Tumorigenic Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with purified recombinant human IL-1 beta (17500 dalton form).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts specifically with IL-1 beta and does not react with IL-1 alpha or with acidic or basic fibroblast growth factor.
It recognizes an epitope distinct from that bound by ILB1-H21 and ILB1-H67 (ATCC HB-10220 and ATCC HB-10222) and that may overlap the epitope bound by ILB1-H34 (ATCC HB-10221).
The affinity constant of ILB1-H6 was measured to be 0.78 X 10 exp10 liter/mol.
Complete Growth Medium The base medium for this cell line is HB101 medium (from Irvine Scientific). To make the complete growth medium, add fetal bovine serum to a final concentration of 7% Alternately, cells may be grown on ATCC Hybri-Care Medium Catalog No. 46-X as the base medium. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. It must be supplemented with 1.5 g/L sodium bicarbonate.
Complete Growth Medium
Culture Medium: Medium HB 101, 93%; fetal bovine serum, 7%. Alternately, cells
may be grown on Hybri-Care, a Modified Dulbecco's medium. Hybri-Care is
available from the ATCC as a powder. A 1-liter package is catalog number 46-X
and five 1-liter packages are catalog number 47-X.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Isotype IgG1; kappa light chain
Name of Depositor Syntex (USA), Inc.
References

Allison AC, et al. Monoclonal antibodies for interleukin-1.beta. US Patent 4,935,343 dated Jun 19 1990

Kenney JS, et al. Monoclonal antibodies to human recombinant interleukin 1 (IL 1)beta: quantitation of IL 1 beta and inhibition of biological activity. J. Immunol. 138: 4236-4242, 1987. PubMed: 2438338

Basic Documentation
References

Allison AC, et al. Monoclonal antibodies for interleukin-1.beta. US Patent 4,935,343 dated Jun 19 1990

Kenney JS, et al. Monoclonal antibodies to human recombinant interleukin 1 (IL 1)beta: quantitation of IL 1 beta and inhibition of biological activity. J. Immunol. 138: 4236-4242, 1987. PubMed: 2438338