Escherichia coli (Migula) Castellani and Chalmers

Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

Certified Reference Material produced under an ISO 17034 accredited process.

1. Open vial according to enclosed instructions.
2. From a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube.  Mix well.
4. Use several drops of this suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate the tubes and plate at 37^oC for 24 hours.

Additional information on this culture is available on the ATCC website at www.atcc.org.

Certified Reference Material produced under an ISO 17034 accredited process.

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