8E5 [derivative of CEM] (ATCC® CRL-8993™)

Organism: Homo sapiens, human  /  Cell Type: lymphoblasthuman immunodeficiency virus (HIV) positive

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Cell Type lymphoblasthuman immunodeficiency virus (HIV) positive
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain RETROVIRUS
Disease acute lymphoblastic leukemia
Age 4 years
Gender female
Ethnicity Caucasian
Applications
No infectious virus is produced; however, co-cultivation with Leu-3 + cells leads to syncytia formation.
Storage Conditions liquid nitrogen vapor phase
Derivation
No infectious virus is produced; however, co-cultivation with Leu-3 + cells leads to syncytia formation.
Clinical Data
female
Caucasian
4 years
Markers
CD4 +; CD5 +
transferrin
Genes Expressed
most human immunodeficiency virus (HIV, HTLV III, LAV) structural proteins,CD4 +; CD5 +
Comments
The cells contain a single defective proviral genome of HTLV III (HIV, LAV).
All of the major structural protein are constitutively expressed with the exception of the p64 and p34 proteins.
No infectious virus is produced; however, co-cultivation with Leu-3 + cells leads to syncytia formation.
The cells have been cured of a mycoplasma contamination.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor The United States of America
References

Powell DM, et al. Cell line producing AIDS viral antigens without producing infectious virus particles. US Patent 4,752,565 dated Jun 21 1988

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Powell DM, et al. Cell line producing AIDS viral antigens without producing infectious virus particles. US Patent 4,752,565 dated Jun 21 1988