2254-62.2 (ATCC® CRL-8544)

Organism: hamster  / 

Organism hamster
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Applications
This line was derived from BHK-21(c-13) (see ATCC CCL-10).
This line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e.
Derivation
This line was derived from BHK-21(c-13) (see ATCC CCL-10).
Comments
This line was derived from BHK-21(c-13) (see ATCC CCL-10).
This line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e.g., Factor VIII).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 100 nM methotrexate, 93%; dialyzed fetal bovine serum, 7%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse with fresh 0.25% trypsin, remove trypsin and incubate the flask at 37C until the cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
Name of Depositor Genentech, Inc.
References

Capon DJ, et al. Preparation of functional human factor VIII in mammalian cells using methotrexate based selection. US Patent 4,965,199 dated Oct 23 1990

Basic Documentation
References

Capon DJ, et al. Preparation of functional human factor VIII in mammalian cells using methotrexate based selection. US Patent 4,965,199 dated Oct 23 1990