2254-62.2 (ATCC® CRL-8544™)

Organism: hamster  / 

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism hamster
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Applications
This line was derived from BHK-21(c-13) (see ATCC CCL-10).
This line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e.
Derivation
This line was derived from BHK-21(c-13) (see ATCC CCL-10).
Comments
This line was derived from BHK-21(c-13) (see ATCC CCL-10).
This line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e.g., Factor VIII).
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse with fresh 0.25% trypsin, remove trypsin and incubate the flask at 37C until the cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
Name of Depositor Genentech, Inc.
References

Capon DJ, et al. Preparation of functional human factor VIII in mammalian cells using methotrexate based selection. US Patent 4,965,199 dated Oct 23 1990

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Capon DJ, et al. Preparation of functional human factor VIII in mammalian cells using methotrexate based selection. US Patent 4,965,199 dated Oct 23 1990