OKT 9 (ATCC® CRL-8021)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Leukemia,Leukemia

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Leukemia,Leukemia
Applications
The antibody reacts primarily with immature thymic lymphocytes and their leukemias and precipitates a 180000 dalton glycoprotein which appears to be the T cell transferrin receptor.
Derivation
Spleen cells were fused with P3X63Ag8.U1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human T cell (activated human T lymphocyte); reacts with transferrin receptor
Cellular Products
immunoglobulin; monoclonal antibody; against human T cell (activated human T lymphocyte); reacts with transferrin receptor
Comments
Animals were immunized with human T cell leukemia cells (acute lymphoblastic leukemia).
Spleen cells were fused with P3X63Ag8.U1 myeloma cells.
The antibody reacts primarily with immature thymic lymphocytes and their leukemias and precipitates a 180000 dalton glycoprotein which appears to be the T cell transferrin receptor.
Complete Growth Medium Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor Ortho Pharmaceutical Corporation
References

Kung PC, Goldstein G. Monoclonal antibody to a human early thymocyte antigen and methods of preparing same. US Patent 4,364,934 dated Dec 21 1982

Sutherland R, et al. Ubiquitous cell-surface glycoprotein on tumor cells is proliferation- associated receptor for transferrin. Proc. Natl. Acad. Sci. USA 78: 4515-4519, 1981. PubMed: 6270680

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kung PC, Goldstein G. Monoclonal antibody to a human early thymocyte antigen and methods of preparing same. US Patent 4,364,934 dated Dec 21 1982

Sutherland R, et al. Ubiquitous cell-surface glycoprotein on tumor cells is proliferation- associated receptor for transferrin. Proc. Natl. Acad. Sci. USA 78: 4515-4519, 1981. PubMed: 6270680