CRL-2955

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Homo sapiens, human
Tissue	lymph node Derived from Metastatic Site: peritoneal effusion
Cell Type	histiocytic
Morphology	lymphoblast-like
Culture Properties	suspension
Biosafety Level	1
Disease	large cell lymphoma; diffuse histiocytic lymphoma
Age	10 years
Gender	Male
Ethnicity	Caucasian
Applications	The availability of a permanently established human neoplastic cell line as the source of this type C RNA virus should greatly facilitate studies of its biological significance. Evidence has recently been obtained that the virus can induce proliferation of normal human monocytes and histiocytes in vitro.
Storage Conditions	liquid nitrogen vapor phase

Karyotype	88 chromosomes; t(2;5)(p23;q35) translocation
Images
Clinical Data	10 years Caucasian Male
Antigen Expression	Monocyte Marker: CD163+ Lymphoid Marker: CD45- Progenitor Markers: CD10-, CD34- Activation Markers: CD30+, CD25+, CD70+, CD71+, CD80-, HLA-DR+, CD45- T-Cell Markers: CD2-, CD3-, CD4-, CD5+, CD7-, CD8- B-Cell Markers: CD19-, CD20-, CD21-, CD22- Myelomonocytic Markers: CD11b-, CD11c-, CD13-, CD14-, CD15-, CD33-
Receptor Expression	CFS-1 expressed IL-1-R, IL-2-Rα, IL-6-R, TNFα-R, & c-fms expressed Surface Receptors: Fc, IgMEAC, IgGEA, & E not expressed
Oncogene	c-fms (proto-oncogene), bcl-6+(c-onc)
Genes Expressed	Fusion gene NPM-ALK (p80)+ anaplastic lymphoma kinase (ALK)+ Ig, not expressed
Comments	The cells are surface Ig negative (sIG-). The cells are non-specific esterase, acid phosphatase and oil red O positive. The cells are periodic acid Schiff negative. These cells phagocytose Candida albicans and latex particles. The cells are reported to be very weakly E - rosette positive. ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Cultures can be maintained by the addition of fresh medium. An inoculum of 8.0 x 10⁴to 2.0 x 10⁵cells/mL is recommended. Subculture when the cell concentration is between 1.0 x 10⁶ and 1.5 x 10⁶ cells/mL. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:12 is recommended. Interval: As needed. Medium renewal: every 2 to 4 days
Cryopreservation	Storage temperature: liquid nitrogen vapor phase Freeze medium: Complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Culture Conditions	Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

STR Profile	D5S818: 11, 12 D13S317: 9, 13 D7S820: 10, 13 D16S539: 11, 12 vWA: 15, 17 THO1: 6, 7 Amelogenin: X Y TPOX: 8 CSF1PO: 12
Population Doubling Capacity	infinite

Name of Depositor	A Epstein
References	Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220 Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039 Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

Name of Depositor

A Epstein

References

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
Other Documentation	Cell Micrograph
References	Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220 Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039 Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

SU-DHL-1 (ATCC® CRL-2955™)

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SU-DHL-1 (ATCC^® CRL-2955^™)