BCL2 (AAA) Jurkat (ATCC® CRL-2902)

Organism: Homo sapiens, human  /  Cell Type: T Cell/ T Lymphocyte  /  Tissue: peripheral blood  /  Disease: acute T cell leukemia

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Tissue peripheral blood
Cell Type T Cell/ T Lymphocyte
Product Format frozen
Morphology Lymphoid-like
Culture Properties suspension
Biosafety Level 2
Disease acute T cell leukemia
Age 14 years
Gender male
Applications

This cell line is useful to study cell apoptosis, BCL-2 function and its pathway, as well as phosphorylation of BCL-2.

Storage Conditions liquid nitrogen vapor phase
Images CRL-2902 Micrograph
Derivation

The BCL2 (AAA) Jurkat cell line was derived by transfecting human Jurkat T cells with the pSFFV-neo mammalian expression vector containing the human BCL2 mutated ORF insert and a neomycin-resistant gene (Parental vector: Addgene # 3349). BCL2 (AAA) stable neomycin (Neo)-resistant single-cell-originated clones were isolated and expanded by selection in medium containing 1 mg/mL G418 for 14 days

Tumorigenic Yes
Comments

The BCL2 (AAA) Jurkat cell line was derived by transfecting human Jurkat T cells with the pSFFV-neo mammalian expression vector containing the human BCL2 mutated ORF insert and a neomycin-resistant gene (Parental vector: Addgene # 3349). The BCL2 (AAA) Jurkat cells contain the BCL2 ORF with a triple sub-mutation at Thr69, Ser70, and Ser87. These amino acids have been replaced with Alanine at their respective positions. BCL2 (AAA) stable neomycin (Neo)-resistant single-cell-originated clones were isolated and expanded by selection in medium containing 1 mg/mL G418 for 14 days. The BCL2 (AAA) Jurkat cell line overexpresses the AA/A-mutated form of Bcl-2.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 10% FBS and 200 mcg/mL G418.
Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Do not allow the cell density to exceed 3 x 106 cells/mL.

Subcultivation ratio: Maintain cultures at a cell concentration between between 1 x 105 and 1 x 106 viable cells/mL.
Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation Freeze medium: 90% Fetal Bovine Serum and 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 9
D13S317: 8, 12
D7S820: 8, 12
D16S539: 11
vWA: 18
TH01: 6, 9.3
Amelogenin: X Y
TPOX: 7, 10, 11
CSF1PO: 11
Name of Depositor Stanley Korsmeyer
Year of Origin 2002
References

Yamamoto K, et al. BCL-2 is Phosphorylated and Inactivated by an ASK1/Jun N-Terminal Protein Kinase Pathway normally Activated at G2/M. Mol Cell Biol. Dec;19(12):8469-78. 1999. PubMed: 10567572

Bassik MC, et al. Phosphorylation of BCL-2 regulates ER Ca2+ homeostasis and apoptosis. Embo J. March 10; 23(5): 1207–1216. 2004. PubMed: 15010700

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Yamamoto K, et al. BCL-2 is Phosphorylated and Inactivated by an ASK1/Jun N-Terminal Protein Kinase Pathway normally Activated at G2/M. Mol Cell Biol. Dec;19(12):8469-78. 1999. PubMed: 10567572

Bassik MC, et al. Phosphorylation of BCL-2 regulates ER Ca2+ homeostasis and apoptosis. Embo J. March 10; 23(5): 1207–1216. 2004. PubMed: 15010700