NL20 (ATCC® CRL-2503)

Organism: Homo sapiens, human  /  Cell Type: Epithelial  /  Tissue: lung/bronchus  /  Disease: Accident victim

Organism Homo sapiens, human
Tissue
lung/bronchus
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 Cells contain SV40 viral sequences
Disease Accident victim
Age 20 years
Gender female
Ethnicity Caucasian, White
Applications
The non-tumorigenic NL20 cell line and the tumorigenic NL20-TA cell line form a pair of immortal cell lines that can be used to study tumor progression.
Storage Conditions liquid nitrogen vapor phase
Karyotype near diploid
Images
Derivation
NL20 (ATCC CRL-2503) is an immortalized, nontumorigenic human bronchial epithelial cell line derived from normal bronchus taken from an accident victim at autopsy.
The cell line was established by transfection with the origin of replication-defective SV40 large T plasmid, p129.
Clinical Data
20 years
Caucasian, White
female
Tumorigenic No
Effects
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments
NL20 cells at passage 183 were inoculated into nude mice and a small slowly growing subcutaneous tumor developed from a minor clone in this otherwise stable cell line.
After three months the tumor was removed and placed in culture. At passage 3, these cells were re-injected into nude mice.
One of the resulting tumors was dissociated, placed in culture and designated NL20-TA. This cell line (ATCC CRL-2504) remains tumorigenic up to at least passage 250.
Complete Growth Medium Ham's F12 medium with 1.5 g/L sodium bicarbonate, 2.7 g/L glucose, 2.0 mM L-glutamine, 0.1 mM nonessential amino acids, 0.005 mg/ml insulin, 10 ng/ml epidermal growth factor, 0.001 mg/ml transferrin, 500 ng/ml hydrocortisone and 4% fetal bovine serum
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:12 to 1: 20 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add dissociation solution (0.02% EDTA and 5% dialized fetal bovine serum in Ca-Mg free Hanks' BSS) and allow the flask to sit at 37C for 12 minutes or until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subculture weekly.
Cryopreservation
Culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor JH Schiller
Passage History
After three months the tumor was removed and placed in culture. At passage 3, these cells were re-injected into nude mice.
NL20 cells at passage 183 were inoculated into nude mice and a small slowly growing subcutaneous tumor developed from a minor clone in this otherwise stable cell line.
One of the resulting tumors was dissociated, placed in culture and designated NL20-TA. This cell line (ATCC CRL-2504) remains tumorigenic up to at least passage 250.
References

Schiller JH, et al. Phenotypic, molecular and genetic characterization of transformed human bronchial epithelial cell strains. Int. J. Oncol. 4: 461-470, 1994.

Schiller JH, Bittner G. Loss of the tumorigenic phenotype with in vitro, but not in vivo, passaging of a novel series of human bronchial epithelial cell lines: possible role of an alpha 5/beta 1-integrin-fibronectin interaction. Cancer Res. : 6215-6221, 1995. PubMed: 8521416

Wittenkeller JL, et al. Comparison of spontaneous and induced mutation rates in an immortalized human bronchial epithelial cell line and its tumorigenic derivative. Oncology 54: 335-341, 1997. PubMed: 9216860

Schiller JH, et al. Karyotypic changes associated with spontaneous acquisition and loss of tumorigenicity in a human transformed bronchial epithelial cell line: evidence for in vivo selection of transformed clones. In Vitro Cell. Dev. Biol. Anim. 34: 283-289, 1998. PubMed: 9590501

Basic Documentation
Other Documentation
References

Schiller JH, et al. Phenotypic, molecular and genetic characterization of transformed human bronchial epithelial cell strains. Int. J. Oncol. 4: 461-470, 1994.

Schiller JH, Bittner G. Loss of the tumorigenic phenotype with in vitro, but not in vivo, passaging of a novel series of human bronchial epithelial cell lines: possible role of an alpha 5/beta 1-integrin-fibronectin interaction. Cancer Res. : 6215-6221, 1995. PubMed: 8521416

Wittenkeller JL, et al. Comparison of spontaneous and induced mutation rates in an immortalized human bronchial epithelial cell line and its tumorigenic derivative. Oncology 54: 335-341, 1997. PubMed: 9216860

Schiller JH, et al. Karyotypic changes associated with spontaneous acquisition and loss of tumorigenicity in a human transformed bronchial epithelial cell line: evidence for in vivo selection of transformed clones. In Vitro Cell. Dev. Biol. Anim. 34: 283-289, 1998. PubMed: 9590501