Human genomic DNA (HCC1954) (ATCC® CRL-2338D)

Organism: Homo sapiens, human  / 

Permits and Restrictions

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Product Format frozen Approx.10 µg
Storage Conditions -20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Introduction

Cell Line Description:

The HCC1954 line is a poorly differentiated cell line initiated on October 30, 1995; it took about 4 months to establish.  HCC1954 was derived from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases.  HCC1954 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for  expression of estrogen receptor (ER) and progesterone receptor (PR). Her2/neu is overexpressed in the ELISA assay.  DNA isolated from an EBV-transformed lymphoblastoid cell line, HCC1954 BL, from the same patient is available as ATCC CRL-2339D.

Quality Control Specifications

DNA Quality Control Information:

·         > 100 kb in size by agarose gel electrophoresis

·         No RNA detected in the agarose gel

·         A260/A280 ratio ³ 1.8

·         Tested and verified for PCR amplification and restriction digestion.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation