SK-PN-DW (ATCC® CRL-2139™)

Organism: Homo sapiens, human  / 

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Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease malignant primitive neuroectodermal tumor
Age 17 years
Gender male
Ethnicity Caucasian
Applications
SK-PN-DW is a primitive neuroectodermal tumor (PNET) cell line derived in 1979 from a 17 year old Caucasian male with embryonal retroperitoneal tumor.
The cells express CCK specific mRNA and synthesize considerable quantities of variably processed CCK prohormone.
Karyotype aneuploid; modal number = 40 to 41; monosomy 13 was found in 100% of the cells; iso(17q) and monosomy 9, 10, 11, and 18 were found in greater than 20% of cells analyzed., In a large proportion of the cells, the double minutes per cell were too numerous to count.
Derivation
SK-PN-DW is a primitive neuroectodermal tumor (PNET) cell line derived in 1979 from a 17 year old Caucasian male with embryonal retroperitoneal tumor.
Clinical Data
SK-PN-DW is a primitive neuroectodermal tumor (PNET) cell line derived in 1979 from a 17 year old Caucasian male with embryonal retroperitoneal tumor.
male
Caucasian
17 years
Genes Expressed
cholecystokinin (CCK)
Tumorigenic Yes
Effects
Yes, forms tumors in nude mice
Comments
SK-PN-DW is a primitive neuroectodermal tumor (PNET) cell line derived in 1979 from a 17 year old Caucasian male with embryonal retroperitoneal tumor.
The cells express CCK specific mRNA and synthesize considerable quantities of variably processed CCK prohormone.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 9
D16S539: 12
D5S818: 13,14
D7S820: 8,14
THO1: 6
TPOX: 8,9
vWA: 16
Name of Depositor C Helson
Year of Origin 1979
References

Potluri VR, et al. Primitive neuroectodermal tumor cell lines: chromosomal analysis of five cases. Cancer Genet. Cytogenet. 24: 75-86, 1987. PubMed: 3024811

Schneider BS, et al. Expression of the cholecystokinin gene by cultured human primitive neuroepithelioma cell lines. J. Clin. Endocrinol. Metab. 69: 411-419, 1989. PubMed: 2753982

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Cancer cell line mutation data
References

Potluri VR, et al. Primitive neuroectodermal tumor cell lines: chromosomal analysis of five cases. Cancer Genet. Cytogenet. 24: 75-86, 1987. PubMed: 3024811

Schneider BS, et al. Expression of the cholecystokinin gene by cultured human primitive neuroepithelioma cell lines. J. Clin. Endocrinol. Metab. 69: 411-419, 1989. PubMed: 2753982