HBE4-E6/E7-C1 [NBE4-E6/E7-C1] (ATCC® CRL-2079™)

Organism: Homo sapiens, human  /  Cell Type: epithelial; human papillomavirus 16 (HPV-16) E6/E7 transforme

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Cell Type epithelial; human papillomavirus 16 (HPV-16) E6/E7 transforme
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 Cells contain human papilloma viral DNA sequences
Age 60 years
Gender male
Ethnicity Caucasian
Karyotype 45, X, -Y, dup (5), -8, +9, -14, -15, -20, -21, -22, +mar1, +mar2, +der(8q;13q)
Derivation
The HBE4-E6/E7-C1 (formerly NBE4-E6/E7-C1) cell line was cloned from passage 22 of HBE4-E6/E7 (see ATCC CRL-2078) by limiting dilution.
Clinical Data
male
Caucasian
60 years
Comments
The HBE4-E6/E7-C1 (formerly NBE4-E6/E7-C1) cell line was cloned from passage 22 of HBE4-E6/E7 (see ATCC CRL-2078) by limiting dilution.
The cloned line is more sensitive than the parental line to induction of terminal differentiation by phorbol esters.
The cells are non-viable in DMSO and should be frozen in culture medium with 10% glycerol.
Complete Growth Medium The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
  • 0.05 mg/ml BPE - provided with the K-SFM kit
  • 5 ng/ml EGF - provided with the K-SFM kit
  • 10 ng/ml cholera toxin - not provided with kit
NOTE: Do not filter complete medium
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  • Remove and discard culture medium.
  • Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution containing 0.5% polyvinylpyrrolidone (PVP).
  • Add 2.0 to 3.0 ml of Trypsin-EDTA-PVP solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  • Add 2.0 to 3.0 ml of complete growth medium containing 0.1% soybean trypsin inhibitor and 0.1% bovine serum albumin and aspirate cells by gently pipetting.Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant.
  • Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
Subculture before the cells become confluent.
Cryopreservation
culture medium, 90%; glycerol, 10%
Population Doubling Time 24 hrs
Name of Depositor J Viallet
Passage History
The HBE4-E6/E7-C1 (formerly NBE4-E6/E7-C1) cell line was cloned from passage 22 of HBE4-E6/E7 (see ATCC CRL-2078) by limiting dilution.
References

Viallet J, et al. Characterization of human bronchial epithelial cells immortalized by the E6 and E7 genes of human papillomavirus type 16. Exp. Cell Res. 212: 36-41, 1994. PubMed: 8174640

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Viallet J, et al. Characterization of human bronchial epithelial cells immortalized by the E6 and E7 genes of human papillomavirus type 16. Exp. Cell Res. 212: 36-41, 1994. PubMed: 8174640