PG-4 (S+L-) (ATCC® CRL-2032)

Organism: Felis catus, cat  /  Cell Type: astrocyte; Moloney murine sarcoma virus (Mo-MSV) transformed  /  Tissue: brain  /  Disease: normal

Permits and Restrictions

View Permits

Organism Felis catus, cat
Tissue brain
Cell Type astrocyte; Moloney murine sarcoma virus (Mo-MSV) transformed
Product Format frozen
Morphology glial, astrocyte
Culture Properties adherent
Biosafety Level 1
Disease normal
Age embryo
Applications
This cell line is useful for detection and quantitation of replication competent retroviruses.
Storage Conditions liquid nitrogen vapor phase
Virus Resistance
murine leukemia virus (MuLV)
Comments
The line is resistant to infection and focus formation by ecotropic MuLV.
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium:
  • heat-inactivated fetal bovine serum to a final concentration of 10%
  • Subculturing
    Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.03% EDTA solution. Remove the trypsin, add fresh trypsin (1 to 2 mL) and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
    Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended
    Medium Renewal: 2 to 3 times per week
    Cryopreservation
    Freeze medium: Complete growth medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
    Name of Depositor KJ Dunn
    Year of Origin May, 1980
    References

    Haapala DK, et al. Isolation from cats of an endogenous type C virus with a novel envelope glycoprotein. J. Virol. 53: 827-833, 1985. PubMed: 2983093

    Bassin RH, et al. Normal DBA/2 mouse cells synthesize a glycoprotein which interferes with MCF virus infection. Virology 123: 139-151, 1982. PubMed: 6959413

    Li Z, et al. PG-4 cell plaque assay for xenotropic murine leukemia virus. J. Virol. Methods 81: 47-53, 1999. PubMed: 10488760

    The cells are useful for detection and assay of various C type retroviruses. PG-4 is one of the cell lines that is recommended by the U.S. Food and Drug Administration for detection of replication competent retroviruses in products and reagents for human use.

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Haapala DK, et al. Isolation from cats of an endogenous type C virus with a novel envelope glycoprotein. J. Virol. 53: 827-833, 1985. PubMed: 2983093

    Bassin RH, et al. Normal DBA/2 mouse cells synthesize a glycoprotein which interferes with MCF virus infection. Virology 123: 139-151, 1982. PubMed: 6959413

    Li Z, et al. PG-4 cell plaque assay for xenotropic murine leukemia virus. J. Virol. Methods 81: 47-53, 1999. PubMed: 10488760

    The cells are useful for detection and assay of various C type retroviruses. PG-4 is one of the cell lines that is recommended by the U.S. Food and Drug Administration for detection of replication competent retroviruses in products and reagents for human use.