T36274 (ATCC® CRL-2012)

Organism: Mus musculus, mouse  /  Cell Type: chemically transformed  / 

Organism Mus musculus, mouse
Cell Type chemically transformed
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age newborn
Strain BALB/c
Applications
This is one of several lines that are useful in studies of the molecular events in tumor promotion and for development of promotion assays.
The T36274 cell line was derived from JB6 Cl 30 by chemical transformation (the cycles of TPA treatment).
Derivation
The T36274 cell line was derived from JB6 Cl 30 by chemical transformation (the cycles of TPA treatment).
Tumorigenic Yes
Effects
Yes, in BALB/c mice and nude mice
Comments
This is one of several lines that are useful in studies of the molecular events in tumor promotion and for development of promotion assays. (see also ATCC CRL-2007 - JB6 Cl 30-7b, ATCC CRL-201 0 - JB6 Cl 41-5a and ATCC CRL-2002 - RT101).
The T36274 cell line was derived from JB6 Cl 30 by chemical transformation (the cycles of TPA treatment).
Complete Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine, 95%; fetal bovine serum, 5%
Subculturing
Subcultivation Ratio: An inoculation density of 2 X 10 exp4 viable cells per 25 sq. cm. flask is recommended
Medium Renewal: 1 to 2 times per week
Remove spent medium, add fresh 0.25% trypsin solution, rinse and remove.
Allow the cells to sit at room temperature for 1 to 3 minutes.
Remove the trypsin and incubate the flask at 37C until the cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
Name of Depositor NH Colburn
References

Colburn NH, et al. A cell culture assay for tumor-promoter-dependent progression toward neoplastic phenotype: detection of tumor promoters and promotion inhibitors. Teratog. Carcinog. Mutagen. 1: 87-96, 1980. PubMed: 6119803

Colburn NH, et al. Correlation of anchorage-independent growth with tumorigenicity of chemically transformed mouse epidermal cells. Cancer Res. 38: 624-634, 1978. PubMed: 626967

Colburn NH, et al. Tumour promoter induces anchorage independence irreversibly. Nature 281: 589-591, 1979. PubMed: 492322

Bernstein LR, Colburn NH. AP1/jun function is differentially induced in promotion-sensitive and resistant JB6 cells. Science 244: 566-569, 1989. PubMed: 2541502

Colburn NH, et al. Dissociation of mitogenesis and late-stage promotion of tumor cell phenotype by phorbol esters: mitogen-resistant variants are sensitive to promotion. Proc. Natl. Acad. Sci. USA 78: 6912-6916, 1981. PubMed: 6947266

Basic Documentation
References

Colburn NH, et al. A cell culture assay for tumor-promoter-dependent progression toward neoplastic phenotype: detection of tumor promoters and promotion inhibitors. Teratog. Carcinog. Mutagen. 1: 87-96, 1980. PubMed: 6119803

Colburn NH, et al. Correlation of anchorage-independent growth with tumorigenicity of chemically transformed mouse epidermal cells. Cancer Res. 38: 624-634, 1978. PubMed: 626967

Colburn NH, et al. Tumour promoter induces anchorage independence irreversibly. Nature 281: 589-591, 1979. PubMed: 492322

Bernstein LR, Colburn NH. AP1/jun function is differentially induced in promotion-sensitive and resistant JB6 cells. Science 244: 566-569, 1989. PubMed: 2541502

Colburn NH, et al. Dissociation of mitogenesis and late-stage promotion of tumor cell phenotype by phorbol esters: mitogen-resistant variants are sensitive to promotion. Proc. Natl. Acad. Sci. USA 78: 6912-6916, 1981. PubMed: 6947266