WM-115 (ATCC® CRL-1675™)

Organism: Homo sapiens, human  /  Tissue: skin  / 

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Organism Homo sapiens, human
Tissue
skin
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age 58 years adult
Gender female
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a hypotetraploid human cell line. The modal chromosome number is 87, occurring at 50%, with higher ploidies at 1.6%. Eight markers [der(1)t(1;9) (p31.2;q11), 6p+, del(6) (p21.1), t(12p14q) and four others] were found in all cells. Of these markers, six (including all four listed above) were consistently paired and two others were single. Normal N6 and N9 were absent and N7 had six copies per cell. There were only two X chromosomes per cell.
Derivation
Another cell line derived from a metastasis in the same patient also is available (ATCC CRL-1676).
The WM-115 cell line was derived from the primary tumor.
Clinical Data
Female
Genes Expressed
proteoglycan antigen characteristic of melanoma
Tumorigenic Yes
Comments
The cells should be incubated at 34°C to 35°C.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.03% EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 34 to 35°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 34 to 35°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze Medium: Complete growth medium 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 34°C; (Max. 35°C, Min. 34°C)
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 12,13
D16S539: 11,12
D5S818: 13
D7S820: 8
THO1: 7,9
TPOX: 8,11
vWA: 15,17
Name of Depositor M Herlyn
Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Cancer Cell Line Mutation Data