VERO 76 (ATCC® CRL-1587)

Organism: Cercopithecus aethiops  /  Tissue: kidney  /  Disease: Normal

Organism Cercopithecus aethiops
Tissue kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease Normal
Age adult
Storage Conditions liquid nitrogen vapor temperature
Derivation
VERO 76 was derived in 1968 by K.M. Johnson and brought to Centers for Disease Control (CDC), Atlanta, GA. It is a derivative of the original VERO.
Comments
When infected with hemorrhagic fever viruses [Machupo (Bolivian), Junin (Argentinian), Lassa (African)], Marburg or Ebola viruses, these cells exhibited cytopathic effects. Plaques are also produced. VERO 76 has a lower saturation density than the original VERO (ATCC CCL-81).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Name of Depositor EM Earley
Year of Origin 1968
References

Earley EM, Johnson KMThe lineage of Vero, Vero 76 and its clone C1008 in the United StatesIn: Earley EM, Johnson KMVero cells: origin, properties and biomedical applicationsTokyoChiba Univ.pp. 26-29, 1988

Policastro PF, et al. Improved plaque assays for Rickettsia prowazekii in Vero76 cells. J. Clin. Microbiol. 34: 1944-1948, 1996. PubMed: 8818887

Schramm N, et al. Vesicles containing Chlamydia trachomatis serovar L2 remain above pH 6 within HEC-1B cells. Infect. Immun. 64: 1208-1214, 1996. PubMed: 8606080

Basic Documentation
References

Earley EM, Johnson KMThe lineage of Vero, Vero 76 and its clone C1008 in the United StatesIn: Earley EM, Johnson KMVero cells: origin, properties and biomedical applicationsTokyoChiba Univ.pp. 26-29, 1988

Policastro PF, et al. Improved plaque assays for Rickettsia prowazekii in Vero76 cells. J. Clin. Microbiol. 34: 1944-1948, 1996. PubMed: 8818887

Schramm N, et al. Vesicles containing Chlamydia trachomatis serovar L2 remain above pH 6 within HEC-1B cells. Infect. Immun. 64: 1208-1214, 1996. PubMed: 8606080