MOLT-3 (ATCC® CRL-1552™)

Organism: Homo sapiens, human  /  Tissue: peripheral blood  /  Cell Type: T lymphoblast

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Homo sapiens, human
Tissue peripheral blood
Cell Type T lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease acute lymphoblastic leukemia
Age 19 years
Gender male
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a hypertetraploid human cell line. The modal chromosome number was 97, occurring in 48% of cells. The rate of cells with higher ploidies was 2%. Three markers were common to all cells. They are: ?del(6) (q21), t(7;7), and an unidentifiable M5. The der(6) generally had three copies per cell, and the i(7q), two copies. The majority of normal chromosomes had 4 copies per cell. N8, N17, N19 and N20 had more than 4 copies in many cells. The X and Y chromosomes were two copies each per cell. Neither HSR chromosomes nor DM's were detected.
Derivation
MOLT-3 was derived from the same patient as the MOLT-4 cell line (ATCC CRL-1582).
This line was established from cells taken from a patient in relapse.
Clinical Data
male
Antigen Expression
CD1 (65%), CD4 (66%), CD5 (97%), CD7 (97%)
Genes Expressed
terminal deoxynucleotidyl transferase (TdT) activity is high,CD1 (65%), CD4 (66%), CD5 (97%), CD7 (97%)
Comments
No immunoglobulin or Epstein-Barr virus is detectable.
The patient had received prior multidrug chemotherapy.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures are maintained by addition or replacement of fresh medium. Establish new cultures at 5 X 105 viable cells/mL and subculture at between 1 and 2 X 106 cells/mL.
Medium Renewal: Every 3 to 4 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Isoenzymes
AK-1, 0
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 0
PGM1, 1
PGM3, 0
Name of Depositor J Minowada
References

Minowada J, et al. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst. 49: 891-895, 1972. PubMed: 4567231

Mertelsmann R, et al. T-cell growth factor (interleukin 2) and terminal transferase activity in human leukemias and lymphoblastic cell lines. Blut 43: 99-103, 1981. PubMed: 6942897

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Minowada J, et al. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst. 49: 891-895, 1972. PubMed: 4567231

Mertelsmann R, et al. T-cell growth factor (interleukin 2) and terminal transferase activity in human leukemias and lymphoblastic cell lines. Blut 43: 99-103, 1981. PubMed: 6942897