HKB-11; Kidney/B Cell Hybrid; Human (Homo sapiens) (CRL-12568)

Designation / Description HKB-11; Kidney/B Cell Hybrid; Human (Homo sapiens)
U.S. Patent Number
Biosafety Level 2 Cells contain adenovirus and EBV viral DNA sequences

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
HKB11 is a derivative of 2B8 (ATCC CRL-12569), a HAT sensitive fusion cell line derived from the P3HR-1 Burkitt's lymphoma cell line (ATCC HTB-62). P3HR1 cells harbor non-immortalizing EBV. P3HR-1 cells were cloned; treated with methanesulfonic acid ethyl ester (MSE) and plated in medium containing 6-thioguanine (6TG). They were selected for deficiency in hypoxanthine-guanine phosphoribosyl transferase (HGPRT-, HPRT-). One of the clones was transfected with the neomycin resistance expression vector pSV2-neo to obtain the 2B8 cell line. A human/human hybrid cell line was made by the fusion of human embryonic kidney cells (293S) and modified Burkitt's lymphoma cells (2B8) to obtain the HKB11 cell line. HKB11 is negative for immunoglobulin gene expression as are 293S cells. HKB11 provides a useful system for mammalian gene expression that is easily adaptable to growth in serum protein-free medium and fermenter/ bioreactor systems.
Shipped frozen
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Cho MS. Human hybrid host cell for mammalian gene expression . US Patent 6,136,599 dated Oct 24 2000

Cho MS, et al. Expression system for factor VIII. US Patent 6,358,703 dated Mar 19 2002

Cho MS, et al. Establishment of a human somatic hybrid cell line for recombinant protein production. J. Biomed. Sci. 9: 631-638, 2002. PubMed: 12432229