H69AR (ATCC® CRL-11351)

Organism: Homo sapiens, human  /  Cell Type: epithelial  /  Tissue: lung  /  Disease: carcinoma; small cell lung cancer; multidrug resistant

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Organism Homo sapiens, human
Tissue lung
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease carcinoma; small cell lung cancer; multidrug resistant
Age 55 years adult
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation

The multiple drug resistant cell line H69AR was established from NCI-H69 cells (ATCC HTB-119) that were grown in the presence of increasing concentrations of adriamycin (doxorubicin) over a total of 14 months.

Clinical Data
55 years adult
Caucasian
male
Tumorigenic Yes
Effects
Yes, in nude mice
Comments

The H69AR cell line is approximately 50-fold resistant to adriamycin as compared to the parental NCI-H69 cell line.

The monoclonal antibodies QCRL-1 (ATCC HB-11765) and QCRL-3 (ATCC HB-11766) will react with fixed H69AR cells.

The cell line is cross-resistant to anthracycline analogues including daunomycin, epirubicin, menogaril, and mitoxantrone as well as to acivicin, etoposide, gramicidin D, colchicine, and the Vinca alkaloids, vincristine and vinblastine.

The cells display little or no cross-resistance to bleomycin, 5-fluorouracil, and carboplatin. They have a slight collateral sensitivity to 1-dehydrotestosterone and lidocaine.

The antibodies reacts with MRP (multidrug resistance protein), a 190,000 integral membrane phosphoglycoprotein that is overexpressed in some drug-selected resistant cell lines

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37oC to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension into new culture vessels.
  6. Incubate cultures at 37oC.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 3 to 5 times per week as culture becomes acidic
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11,13
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17
Population Doubling Time 24 hrs
Name of Depositor S Cole
References

Deeley RG, Cole SP. Antibodies to a multidrug resistance protein. US Patent 6,063,621 dated May 16 2000

Hipfner DR, et al. Detection of the M(r) 190,000 multidrug resistance protein, MRP, with monoclonal antibodies. Cancer Res. 54: 5788-5792, 1994. PubMed: 7954400

Mirski SE, et al. Multidrug resistance in a human small cell lung cancer cell line selected in adriamycin. Cancer Res. 47: 2594-2598, 1987. PubMed: 2436751

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Deeley RG, Cole SP. Antibodies to a multidrug resistance protein. US Patent 6,063,621 dated May 16 2000

Hipfner DR, et al. Detection of the M(r) 190,000 multidrug resistance protein, MRP, with monoclonal antibodies. Cancer Res. 54: 5788-5792, 1994. PubMed: 7954400

Mirski SE, et al. Multidrug resistance in a human small cell lung cancer cell line selected in adriamycin. Cancer Res. 47: 2594-2598, 1987. PubMed: 2436751