Cri du Chat (ATCC® CCL-90)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age adult
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype stable with deletion in chromosome 5 characteristic of syndrome
Clinical Data
female
Caucasian
adult
Virus Susceptibility Human poliovirus 1
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: At the time of subcultivation (every 5 to 7 days)
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10,11
D7S820: 8,11
THO1: 9.3
TPOX: 8
vWA: 15,16
Name of Depositor WR Breg
Year of Origin February, 1967
References

Miller OJ, et al. Alternative DNA replication patterns associated with long arm length of chromosomes 4 and 5 in the cri du chat syndrome. Cytogenetics 5: 137-151, 1966. PubMed: 5921496

Basic Documentation
References

Miller OJ, et al. Alternative DNA replication patterns associated with long arm length of chromosomes 4 and 5 in the cri du chat syndrome. Cytogenetics 5: 137-151, 1966. PubMed: 5921496