RTG-2 (ATCC® CCL-55)

Organism: Oncorhynchus mykiss, trout, rainbow  /  Tissue: mixed; testis; ovary  / 

Permits and Restrictions

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Organism Oncorhynchus mykiss, trout, rainbow
Tissue mixed; testis; ovary
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Gender male and female mixed
Storage Conditions liquid nitrogen vapor phase
Virus Resistance
poliovirus 1
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with cold (less than 24°C) 0.25% (w/v) Trypsin - 0.03% EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.Cultures may be established with as few as 3 x 103 cells/mL.
  6. Incubate cultures at 22°C.

Medium Renewal: Once per week

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells: A Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley - Liss, N.Y., 2005.

Culture Conditions Temperature: 22°C; cells do not survive above 26°C but will grow at temperatures as low as 4°C.
Name of Depositor K Wolf
References

. . Science 135: 1065, 1962.

Officer JE. Ability of a fish cell line to support the growth of mammalian viruses. Proc. Soc. Exp. Biol. Med. 116: 190-194, 1964. PubMed: 14200103

Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741

Cross References

Nucleotide (GenBank) : U82129 Oncorhynchus mykiss Y1 RNA, partial sequence.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. . Science 135: 1065, 1962.

Officer JE. Ability of a fish cell line to support the growth of mammalian viruses. Proc. Soc. Exp. Biol. Med. 116: 190-194, 1964. PubMed: 14200103

Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741