NCI-H508 [H508] (ATCC® CCL-253™)

Organism: Homo sapiens, human  /  Tissue: Cecum  / 

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Organism Homo sapiens, human
Tissue Cecum
Product Format frozen
Morphology epithelial
Culture Properties floating aggregates of round cells with some attached cells
Biosafety Level 1
Disease colorectal adenocarcinoma
Age 55 years
Gender male
Ethnicity Caucasian
Karyotype modal number = 102; range = 71 to 131; most cells have DM's
Derivation
This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil.
Clinical Data
55 years
Caucasian
male
Antigen Expression
Blood type A; Rh+; CA19-9 antigen
Genes Expressed
carcinoembryonic antigen (CEA), 1709 ng/mL per 106 cells per 10 days,Blood type A; Rh+; CA19-9 antigen
Tumorigenic Yes
Comments The cells contain Dopa decarboxylase, CA19-9 antigen and CEA but do not express the TAG-72 antigen.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 3 times per week

Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution for two minutes. Remove the trypsin and let the flask sit at room temperature for 10 minutes (or until the cells detach). Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12
D5S818: 12
D7S820: 8,12
THO1: 9,9.3
TPOX: 8,11
vWA: 15,16
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Name of Depositor AF Gazdar
References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Cancer cell line mutation data
References

Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells