LoVo (ATCC® CCL-229)

Organism: Homo sapiens, human  /  Tissue: colon; derived from metastatic site: left supraclavicular region  /  Disease: Dukes' type C, grade IV, colorectal adenocarcinoma

Organism Homo sapiens, human
Tissue colon; derived from metastatic site: left supraclavicular region
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease Dukes' type C, grade IV, colorectal adenocarcinoma
Age 56 years
Gender male
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype The stemline chromosome number is hyperdiploid with the 2S component occurring at about 2.7% and 3 marker chromosomes were common to all S metaphases. Karyotypes were generally homogeneous and stable.
Images
Derivation
LoVo was initiated in 1971 from a fragment of a metastatic tumor nodule in the left supraclavicular region of a 56-year-old Caucasian male patient with a histologically proven diagnosis of adenocarcinoma of the colon.
Clinical Data
male
Antigen Expression HLA A11, B15, B17, Cw1, Cw3; blood type B
Oncogene myc +; myb + ; ras +; fos +; p53 +; sis -; abl -; ros -; src -
Genes Expressed
carcinoembryonic antigen (CEA) 908 ng/10 6 cells/10 days.  The cells are negative for expression of CSAp (CSAp-) and colon antigen 3. 
Cellular Products
carcinoembryonic antigen (CEA) 908 ng/10 exp6 cells/10 days
Tumorigenic Yes
Effects
Yes, in nude mice
Comments
The cells are negative for expression of CSAp (CSAp-) and colon antigen 3.

The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes.

N-myc and sis oncogene expression were not detected.

Tumor specific nuclear matrix proteins CC-3 and CC-4 are expressed.

Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM  EDTA solution to remove all traces of serum, which  contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is  dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate  cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: XY
CSF1PO: 10,11,13,14
D13S317: 8, 11
D16S539: 9, 12
D5S818: 11, 12, 13
D7S820: 9.3,10, 11
THO1: 9.3
TPOX: 8,9
vWA: 17,18
Isoenzymes
ES-D, 1
G6PD, B
PGD, A
PGM1, 2
PGM3, 1-2
Name of Depositor M Romsdahl
Year of Origin 1971
References

Drewinko B, et al. Further biologic characteristics of a human carcinoembryonic antigen-producing colon carcinoma cell line. J. Natl. Cancer Inst. 61: 75-83, 1978. PubMed: 276641

Drewinko B, Yand LY. Restriction of CEA synthesis to the stationary phase of growth of cultured human colon carcinoma cells. Exp. Cell Res. 101: 414-416, 1976. PubMed: 964319

Drewinko B, et al. Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line. Cancer Res. 36: 467-475, 1976. PubMed: 1260746

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Keesee SK, et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905

Drewinko B, et al. Response of exponentially growing, stationary-phase, and synchronized cultured human colon carcinoma cells to treatment with nitrosourea derivatives. Cancer Res. 39: 2630-2636, 1979. PubMed: 445465

Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538

Cross References

Nucleotide (GenBank) : X17097 Human PSG9 mRNA for pregnancy specific glycoprotein 9.

Nucleotide (GenBank) : X17098 Human PSG10 mRNA for pregnancy specific glycoprotein 10.

Nucleotide (GenBank) : X14831 Human mRNA for transmembrane carcinoembryonic antigen BGPb (formerly TM2-CEA).

Basic Documentation
Other Documentation
References

Drewinko B, et al. Further biologic characteristics of a human carcinoembryonic antigen-producing colon carcinoma cell line. J. Natl. Cancer Inst. 61: 75-83, 1978. PubMed: 276641

Drewinko B, Yand LY. Restriction of CEA synthesis to the stationary phase of growth of cultured human colon carcinoma cells. Exp. Cell Res. 101: 414-416, 1976. PubMed: 964319

Drewinko B, et al. Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line. Cancer Res. 36: 467-475, 1976. PubMed: 1260746

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Keesee SK, et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905

Drewinko B, et al. Response of exponentially growing, stationary-phase, and synchronized cultured human colon carcinoma cells to treatment with nitrosourea derivatives. Cancer Res. 39: 2630-2636, 1979. PubMed: 445465

Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538