COLO 320DM (ATCC® CCL-220)

Organism: Homo sapiens, human  /  Tissue: colon  /  Disease: Dukes' type C, colorectal adenocarcinoma

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Organism Homo sapiens, human
Tissue colon
Product Format frozen
Morphology rounded and refractile
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Disease Dukes' type C, colorectal adenocarcinoma
Age 55 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 24; range = 49 to 61.
The stemline chromosome number is hyperdiploid and 9-11 marker chromosomes (M1, M2, M3, M5, M10, M11, M12, M13, M14, 7p- and 10 q+) were common. Double minutes were observed in 24% of the metaphases examined.
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Clinical Data
55 years
Caucasian
female
Genes Expressed
serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone
Tumorigenic Yes
Effects
Yes, in nude mice
Comments
The line is negative for CEA, CSAp and colon antigen 3.
The cells are weakly positive for keratins and vimentin.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol.
  1. Add 2.0 to 3.0 mL of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.
  2. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  3. Add appropriate aliquots of the cell suspension to new culture vessels.
  4. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11,12
D5S818: 12
D7S820: 9,12
THO1: 9
TPOX: 8,9
vWA: 15,18
Isoenzymes
ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 2
Name of Depositor GE Moore
References

Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874