D-17 (ATCC® CCL-183)

Organism: Canis familiaris  /  Tissue: bone; derived from metastatic site: lung  /  Disease: osteosarcoma

Organism Canis familiaris
Tissue bone; derived from metastatic site: lung
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease osteosarcoma
Age 11 years
Gender female
Strain poodle
Applications
This cell line may be used as a transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 63; range = 58 to 70.
There were 25 to 30 biarmed chromosomes not common to the diploid dog karyotype present in all cells examined. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines
Derivation
Derived from an osteosarcoma metastatic to the lung in an 11-year-old female poodle.
Tumorigenic Yes
Effects
Yes, in immunosuppressed mice
Yes, in nude mice
Virus Susceptibility Canine adenovirus 1
Canine herpesvirus 1 , Canine herpesvirus
Canine parainfluenza virus
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor WA Nelson-Rees
Year of Origin 1969
References

Temin HM, Watanabe S. Helper cell. US Patent 4,650,764 dated Mar 17 1987

Riggs JL, et al. Immunofluorescent studies of RD-114 virus replication in cell culture. J. Gen. Virol. 25: 21-29, 1974. PubMed: 4372315

Basic Documentation
References

Temin HM, Watanabe S. Helper cell. US Patent 4,650,764 dated Mar 17 1987

Riggs JL, et al. Immunofluorescent studies of RD-114 virus replication in cell culture. J. Gen. Virol. 25: 21-29, 1974. PubMed: 4372315