RD ATCC ® CCL-136™ Homo sapiens muscle rhabdomyosarcoma

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Homo sapiens, human
Tissue	muscle
Product Format	frozen
Morphology	spindle cells and large multinucleated cells
Culture Properties	adherent
Biosafety Level	1
Disease	rhabdomyosarcoma
Age	7 years
Gender	female
Ethnicity	Caucasian
Applications	This cell line can be used for the detection of viruses.
Storage Conditions	liquid nitrogen vapor temperature

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Clinical Data	7 years Caucasian female
Genes Expressed	myoglobin,myosin ATPase

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 3 to 4 days
Cryopreservation	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature
Culture Conditions	Temperature: 37°C

STR Profile	Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 10,11 D5S818: 11 D7S820: 8,12 THO1: 9.3 TPOX: 9 vWA: 18
Isoenzymes	G6PD, B

Name of Depositor	RM McAllister
References	American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992. McAllister RM, et al. Cultivation in vitro of cells derived from a human rhabdomyosarcoma. Cancer 24: 520-526, 1969. PubMed: 4241949 Chen TR, et al. Cell identity resolved. Nature 340: 106, 1989. PubMed: 2739733 Stratton MR, et al. Misidentified cell. Nature 337: 311-312, 1989. PubMed: 2911385 Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033 Hahon N, Cooke KO. Primary virus-cell interactions in the immunofluorescence assay of Venezuelan equine encephalomyelitis virus. J. Virol. 1: 326, 1967. PubMed: 4918236
Cross References	Nucleotide (GenBank) : U09279 Human type XIX collagen (COL19A1) mRNA, partial cds.

Name of Depositor

RM McAllister

References

American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.

McAllister RM, et al. Cultivation in vitro of cells derived from a human rhabdomyosarcoma. Cancer 24: 520-526, 1969. PubMed: 4241949

Chen TR, et al. Cell identity resolved. Nature 340: 106, 1989. PubMed: 2739733

Stratton MR, et al. Misidentified cell. Nature 337: 311-312, 1989. PubMed: 2911385

Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033

Hahon N, Cooke KO. Primary virus-cell interactions in the immunofluorescence assay of Venezuelan equine encephalomyelitis virus. J. Virol. 1: 326, 1967. PubMed: 4918236

Cross References

Nucleotide (GenBank) : U09279 Human type XIX collagen (COL19A1) mRNA, partial cds.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
Other Documentation	Cell Micrograph Cell Micrograph Cancer cell line mutation data
References	American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992. McAllister RM, et al. Cultivation in vitro of cells derived from a human rhabdomyosarcoma. Cancer 24: 520-526, 1969. PubMed: 4241949 Chen TR, et al. Cell identity resolved. Nature 340: 106, 1989. PubMed: 2739733 Stratton MR, et al. Misidentified cell. Nature 337: 311-312, 1989. PubMed: 2911385 Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033 Hahon N, Cooke KO. Primary virus-cell interactions in the immunofluorescence assay of Venezuelan equine encephalomyelitis virus. J. Virol. 1: 326, 1967. PubMed: 4918236

RD (ATCC® CCL-136™)

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RD (ATCC^® CCL-136^™)