CCRF-CEM [CCRF CEM] (ATCC® CCL-119)

Organism: Homo sapiens, human  /  Cell Type: T lymphoblast  /  Tissue: peripheral blood  /  Disease: acute lymphoblastic leukemia

Organism Homo sapiens, human
Tissue peripheral blood
Cell Type T lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease acute lymphoblastic leukemia
Age 4 years juvenile
Gender female
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 47; range = 41 to 95.
Fifty karyotypes showed no consistent loss or gain of particular chromosomes. Twenty-eight percent of the cells with 45 chromosomes were C-; 53% of all cells had an extra D and 35% had an extra F. Only N1 and N18 were not affected by gain or loss. No marker chromosomes noted.
Images
Derivation Human lymphoblasts from peripheral blood of a child with acute leukemia.
Clinical Data
Caucasian
female
4 years juvenile
Antigen Expression
CD3; Homo sapiens, expressed
CD4; Homo sapiens, expressed
CD5; Homo sapiens, expressed
CD7; Homo sapiens, expressed
Genes Expressed
CD3; Homo sapiens, expressed ,CD5; Homo sapiens, expressed ,CD7; Homo sapiens, expressed ,CD4; Homo sapiens, expressed
Tumorigenic Yes
Effects
Yes, in syrian hamsters
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 105 viable cells/mL. Maintain cell density between 2 to 3 X 105 and 1 to 2 X 106 viable cells/mL.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 7.5% DMSO. 
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 10,13
D5S818: 12,13
D7S820: 9,13
THO1: 6,7
TPOX: 8
vWA: 17,19
Isoenzymes
ADA, 1
ES-D, 1
G6PD, B
GLO-I, 1
PEP-D, 1
PGD, C
PGM1, 1
PGM3, 0
Name of Depositor GE Foley
Year of Origin November, 1964
References

Foley GE, et al. Continous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522-529, 1965. PubMed: 14278051

Sandstrom PA, Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. PubMed: 8506323

Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381

Uzman BG, et al. Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19: 1725-1742, 1966. PubMed: 5224274

Adams RA, et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster. Cancer Res. 27: 772-783, 1967. PubMed: 4295047

Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538

CCRF-CEM is a T lymphoblastoid cell line derived by G.E. Foley, et al. using cells obtained in November, 1964 from peripheral blood buffy coat of a 4-year-old Caucasian female with acute lymphoblastic leukemia.

Cross References

Nucleotide (GenBank) : Z23090 H.sapiens mRNA for 28 kDa heat shock protein.

Nucleotide (GenBank) : AF146431 Homo sapiens cell-line CCRF-CEM sodium/hydrogen exchanger isoform 1 mRNA, complete cds.

Nucleotide (GenBank) : Z12846 HSA08C081 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 08C08, mRNA sequence.

Nucleotide (GenBank) : Z13104 HSA25H071 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 25H07, mRNA sequence.

Nucleotide (GenBank) : Z13398 HSA45A081 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 45A08, mRNA sequence.

Nucleotide (GenBank) : Z13431 HSA48B021 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 48B02, mRNA sequence.

Nucleotide (GenBank) : Z13562 HSA57F051 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 57F05, mRNA sequence.

Nucleotide (GenBank) : Z13677 HSA65D041 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 65D04, mRNA sequence.

Nucleotide (GenBank) : Z13711 HSA68F071 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 68F07, mRNA sequence.

Nucleotide (GenBank) : Z13717 HSA69C041 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 69C04, mRNA sequence.

Nucleotide (GenBank) : Z15500 HSA23D012 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 23D01, mRNA sequence.

Nucleotide (GenBank) : Z15555 HSA27A032 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 27A03, mRNA sequence.

Nucleotide (GenBank) : Z16011 HSA56G072 CLONTECH cDNA library CCRF-CEM, cat# HL1063g Homo sapiens cDNA clone 56G07, mRNA sequence.

Basic Documentation
Other Documentation
FAQ's
  1. Normal Morphology of ATCC® CCL-119
    ATCC® CCL-119
    Date Updated: 7/25/2012
References

Foley GE, et al. Continous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522-529, 1965. PubMed: 14278051

Sandstrom PA, Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. PubMed: 8506323

Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381

Uzman BG, et al. Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19: 1725-1742, 1966. PubMed: 5224274

Adams RA, et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster. Cancer Res. 27: 772-783, 1967. PubMed: 4295047

Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538

CCRF-CEM is a T lymphoblastoid cell line derived by G.E. Foley, et al. using cells obtained in November, 1964 from peripheral blood buffy coat of a 4-year-old Caucasian female with acute lymphoblastic leukemia.