A9 (APRT and HPRT negative derivative of Strain L) (ATCC® CCL-1.4™)

Organism: Mus musculus, mouse  /  Tissue: subcutaneous connective tissue; areolar and adipose  / 

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Mus musculus, mouse
Tissue subcutaneous connective tissue; areolar and adipose
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 100 days
Gender male
Strain C3H/An
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 52; range = 46 to 54; This is a hyperdiploid cell line with 24 to 26 marker chromosomes common to all cells., Nine markers (M1 - M9) are common to L-M(TK-) (see ATCC CCL-1.3) and 16 to 17 are found only in A9. The latter markers include Rb(3.5), Rb(4.15), Rb(5.5), T(8;19), Rb(9.17) and 11 to 12 others., Structurally normal chromosomes 4, 15, 16, 17, 18 and X were absent.
Derivation
The A9 cell line was isolated by J.W. Littlefield from wild-type Strain L cells.
Clinical Data
male
Tumorigenic Yes
Comments

The cells are sensitive to HAT selection medium and deficient in adenosine phosphoribosyl transferase (APRT) and hypoxanthine phosphoribosyl transferase (HPRT).

The cells are resistant to 8-azaguanine (0.003 mg/mL) and 6-thioguanine (0.1 mM).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5g/L glucose, 90%; fetal bovine serum, 10%
Subculturing

Remove old medium, add fresh 0.25% trypsin, EDTA (0.03%) solution, rinse and remove. Allow cells to sit at room temperature (or at 37°C) until cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days
Name of Depositor TR Chen
References

Littlefield JW. The inosinic acid pyrophosphorylase activity of mouse fibroblasts partially resistant to 8-azaguanine. Proc. Natl. Acad. Sci. USA 50: 568-573, 1963. PubMed: 14067106

Chen TR. Cytogenetics of somatic cell hybrids. II. Modal karyotypes and mosaicism in mouse mutant cell lines: LM(tk-), A9, and Rag. Cancer Genet. Cytogenet. 24: 95-107, 1987. PubMed: 3466673

Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Littlefield JW. The inosinic acid pyrophosphorylase activity of mouse fibroblasts partially resistant to 8-azaguanine. Proc. Natl. Acad. Sci. USA 50: 568-573, 1963. PubMed: 14067106

Chen TR. Cytogenetics of somatic cell hybrids. II. Modal karyotypes and mosaicism in mouse mutant cell lines: LM(tk-), A9, and Rag. Cancer Genet. Cytogenet. 24: 95-107, 1987. PubMed: 3466673

Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365