Clostridium difficile (Hall and O'Toole) Prevot (ATCC® BAA-1382)

Strain Designations: 630  /  Type Strain: no  /  Biosafety Level: 2

Strain Designations 630
Application
Enteric Research
Isolation
Clincal isolate, Switzerland
Type Strain no
Biosafety Level 2
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype Toxinotype: 0 RefRupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999
Genome Sequenced Strain

Clostridium difficile 630, complete genome

Type Strain no
Comments
Genome sequenced strain
Presence of tcdA and tcdB genes confirmed by PCR. RefLemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303
Binary toxin gene cdtB not amplified by PCR
Ribotype 012
Toxinotype 0
Medium ATCC® Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic gas mixture, 80% N2 -10% CO2 -10% H2
Genotype Toxinotype: 0 RefRupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999
Name of Depositor B Wren, H Maschler
Isolation
Clincal isolate, Switzerland
References

Wust Jurg, et al. Investigation of an Outbreak of Antibiotic-Associated Colitis by Various Typing Methods. J. Clin. Microbiol. 16: 1096-1101, 1982.

Sebaihia Mohammed. The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome. Nat. Genet. 38: 779-786, 2006.

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Cross References

Nucleotide (Genbank) : NC_009089 Clostridium difficile 630, complete genome

Nucleotide (GenBank) : NC_008226 Clostridium difficile 630 plasmid pCD630, complete sequence.

Nucleotide (GenBank) : AM180355 Clostridium difficile 630 complete genome.