pCM189 (ATCC® 87661)

Applications: YC-type (centromeric) shuttle vectorexpression vector  /  Depositors: E Herrero

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Designations pCM189
Depositors E Herrero
Biosafety Level 1
Host E. coli DH5α
Vector Information
Size (kb): 8.37
Vector: pCM189 (plasmid)
Promoters: Promoter CMV
Marker(s): URA3,ampR
Construct size (kb): 8.37
Features: marker(s): URA3
marker(s): ampR
operator: tetO - seven repeats
other: tTA transcriptional activator
promoter: CMV
promoter: CYC1
replicon: ARS1
replicon: pMB1
MCS: BamHI...PstI
terminator: ADH1
terminator: CYC1
centromere: CEN4
Applications
YC-type (centromeric) shuttle vector
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--8.4; PstI--8.4; BamHI/XhoI--7.7, 0.67.
Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Gari E, et al. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast 13: 837-848, 1997. PubMed: 9234672

Related Products
Component of: ATCC 87663
Shipped frozen
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