Size (kb): 7.4000000953674320
Vector: pSLF102 (plasmid)
Promoters: Promoter CaMV
Construct size (kb): 7.400000095367432
Features: marker(s): ampR
Restriction digests of the clone give the following sizes (kb): BamHI--7.4; HindIII--5.6, 1.8; SmaI--7.4.
The vector contains the constitutive CaMV promoter adjacent to the tet operator.
The vectors pSLF101 and pSLF102 differ in that pSLF101 has LEU2 marker and pSLF102, ura4+.
A tet repressor must be supplied to regulate expression. Schizosaccharomyces pombe FY191 (ATCC 201437)
has a tet repressor gene and integrating vector pSLF104 (ATCC 87621)
contains the sup3-5 marker and tet repressor under adh promoter.
Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516