pEA2 (ATCC® 87617)

Applications: contains easily purifiable cassette(s) for construction  /  Depositors: SL Forsburg

Permits and Restrictions

View Permits

Designations pEA2
Depositors SL Forsburg
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.8499999046325680
Vector: pEA2 (plasmid)
Construction: pUC18, pMN1
Marker(s):ampR,his7+
Construct size (kb): 5.849999904632568
Features: marker(s): ampR
marker(s): his7+
replicon: pMB1
MCS: SphI...EcoRI
Applications
contains easily purifiable cassette(s) for construction
Comments
Restriction digests of the clone give the following sizes (kb): XbaI--4.5, 1.4; HindIII--3.8, 1.3, 0.80; PstI--5.9.
pEA2 carries his7+ cloned into the pUC18 polylinker. To construct gene disruptions from this vector, fragments carrying his7+ can be isolated using a variety of restriction enzymes.
Constructed by inserting the 3.15 kb BglII fragment from plasmid pMN1 into the BamHI site of pUC18.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Apolinario E, et al. Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies. Curr. Genet. 24: 491-495, 1993. PubMed: 8299169

Related Products
component of:ATCC 87686
Shipped freeze-dried
Shipping Information

 Freeze dried E. coli containing the plasmid

Product Sheet
Product Sheet
Product Sheet