pEA2 (ATCC® 87617)

Applications: contains easily purifiable cassette(s) for construction  /  Depositors: SL Forsburg

Permits and Restrictions

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Designations pEA2
Depositors SL Forsburg
Biosafety Level 1
Vector Information
Size (kb): 5.8499999046325680
Vector: pEA2 (plasmid)
Construction: pUC18, pMN1
Marker(s):ampR,his7+
Construct size (kb): 5.849999904632568
Features: marker(s): ampR
marker(s): his7+
replicon: pMB1
MCS: SphI...EcoRI
Applications
contains easily purifiable cassette(s) for construction
Comments
Restriction digests of the clone give the following sizes (kb): XbaI--4.5, 1.4; HindIII--3.8, 1.3, 0.80; PstI--5.9.
pEA2 carries his7+ cloned into the pUC18 polylinker. To construct gene disruptions from this vector, fragments carrying his7+ can be isolated using a variety of restriction enzymes.
Constructed by inserting the 3.15 kb BglII fragment from plasmid pMN1 into the BamHI site of pUC18.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Apolinario E, et al. Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies. Curr. Genet. 24: 491-495, 1993. PubMed: 8299169

Related Products
component of:ATCC 87686
Shipped freeze-dried
Shipping Information

 Freeze dried E. coli containing the plasmid

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