YIplac211 (ATCC® 87593)

Applications: YI-type (integrating) shuttle vectorvector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: RD Gietz

Permits and Restrictions

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Designations YIplac211
Depositors RD Gietz
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.797
Vector: YIplac211 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):URA3,ampR
Construct size (kb): 3.797
Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: lac
replicon: pMB1
MCS: HindIII...EcoRI
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII
Other unique sites: EcoRV AatII SspI NarI NsiI StuI ApaI NcoI
Applications
YI-type (integrating) shuttle vector
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): BglI--2.2, 1.6; PvuI--2.1, 1.8; PstI--3.9
One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1).
The PstI restriction site from the URA3 gene of S. cerevisiae was removed by using oligo-directed mutagenesis.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106

Related Products
component of:ATCC 87623
component of:ATCC 87638
Shipped frozen
Shipping Information

 Frozen glycerol stock of E. coli containing the plasmid

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Attachments Vector Map