YIplac211 (ATCC® 87593)

Applications: YI-type (integrating) shuttle vectorvector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: RD Gietz

Permits and Restrictions

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Designations YIplac211
Depositors RD Gietz
Biosafety Level 1
Vector Information
Size (kb): 3.797
Vector: YIplac211 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):URA3,ampR
Construct size (kb): 3.797
Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: lac
replicon: pMB1
MCS: HindIII...EcoRI
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI HindIII
Other unique sites: EcoRV AatII SspI NarI NsiI StuI ApaI NcoI
Applications
YI-type (integrating) shuttle vector
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): BglI--2.2, 1.6; PvuI--2.1, 1.8; PstI--3.9
One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1).
The PstI restriction site from the URA3 gene of S. cerevisiae was removed by using oligo-directed mutagenesis.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106

Related Products
component of:ATCC 87623
component of:ATCC 87638
Shipped frozen
Shipping Information

 Frozen glycerol stock of E. coli containing the plasmid

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