YCplac33 (ATCC® 87586)

Applications: YC-type (centromeric) shuttle vectorvector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: RD Gietz

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Designations YCplac33
Depositors RD Gietz
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.6030001640319820
DESCRIPTION OF VECTOR:
Intact vector size: 5.603
Type of vector: plasmid
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Other unique sites: EcoRV NdeI AatII NarI SpeI NaeI NheI BglII ClaI NsiI StuI
Construction: pUC19
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
promoter: lac, ->, 143-171
insert detection: lacZ', ->, 216-500
MCS: HindIII...EcoRI, ->, 234-285
centromere: CEN4, 504-1480
replicon: ARS1, 1481-2225
marker(s): URA3, <-, 2563-3365
marker(s): ampR, ->, 3802-4662
replicon: pMB1, 5420-5420
Vector: YCplac33 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):URA3,ampR
Construct size (kb): 5.603000164031982
Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: pMB1
MCS: HindIII...EcoRI
centromere: CEN4
Applications
YC-type (centromeric) shuttle vector
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): AvaI--3.7, 1.9; ClaI--5.7; PstI--5.6.
One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1).
The PstI restriction site from the URA3 gene of S. cerevisiae were removed by using oligo-directed mutagenesis.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106

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Shipped frozen
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87586